Bacillus Sp. Mediated Extracellular Synthesis of Silver Nanoparticles
نویسندگان
چکیده
Objective: To synthesize silver nanoparticles extracellularly using Bacillus sp. isolated from soil sample. Methods: Bacteria capable of synthesizing silver nanoparticles extracellularly were screened by treating culture supernatant with silver nitrate solution. The potential isolate was isolated on nutrient agar medium and identified by Gram staining and biochemical tests. Results: One of the isolates was found to be potential in extracellular synthesis of silver nanoparticle. The isolate was identified as Bacillus sp. The biosynthesized silver nanoparticles were characterized by using UV-Visible spectroscopy, X-ray diffraction analysis, Scanning Electron Microscopy and Atomic Force Microscopic analysis. The biosynthesized silver nanoparticles were found to be spherical with 50-100 nm diameter. Conclusion: Mono-dispersed silver nanoparticles in the range of 50 to 120 nm was synthesised using culture supernatant of newly isolated Bacillus sp.
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